stressmarq spc 404 (StressMarq)

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StressMarq stressmarq spc 404
Stressmarq Spc 404, supplied by StressMarq, used in various techniques. Bioz Stars score: 93/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spc+404d/ENaC+beta+Antibody/pm41930611-90-58-58
Average 93 stars, based on 87 article reviews

stressmarq spc 404 - by Bioz Stars, 2026-10

93/100 stars

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Article Title: SPAK and OSR1 play essential roles in potassium homeostasis through actions on the distal convoluted tubule
Article Snippet: Commercial antibodies were used against β‐actin (1:5000) (ab8227, Abcam, Cambridge, MA, USA), ENaC‐Beta (1:1000) (SPC‐404D, StressMarq, Victoria, BC, Canada), ENaC‐Gamma (1:1000) (SPC‐405D, StressMarq), pT197‐PKA catalytic subunit (1:2500) (ab75991, Abcam), pT172‐AMPK (1:1000) (2531S, Cell Signaling, Danvers, MA, USA), MO25‐alpha (1:1000) (2716, Cell Signaling).

Article Title: SPAK and OSR1 play essential roles in potassium homeostasis through actions on the distal convoluted tubule
Article Snippet: Commercial antibodies were used against β-actin (1:5000) (ab8227, Abcam, Cambridge, MA, USA), ENaC-Beta (1:1000) (SPC-404D, StressMarq, Victoria, BC, Canada), ENaC-Gamma (1:1000) (SPC-405D, StressMarq), pT197-PKA catalytic subunit (1:2500) (ab75991, Abcam), pT172-AMPK (1:1000) (2531S, Cell Signaling, Danvers, MA, USA), MO25-alpha (1:1000) (2716, Cell Signaling).

Saline:

Article Title: (Pro)Renin receptor regulates potassium homeostasis through a local mechanism
Article Snippet: Samples were resolved by SDS-PAGE and transferred onto polyvinylidene fluoride membrane (Immobilion-P, Millipore, Bedford, MA). .. The membranes were blocked with 5% nonfat dry milk in Tris-buffered saline with Tween-20 (TBST) for 1 h at room temperature, followed by incubation with primary antibodies [PRR: 1:1,000 dilution, ab40790; Abcam, Cambridge, MA; NCC: 1:1000 dilution, SPC-402D, StressMarq, Victoria, BC, Canada; α-epithelial sodium channel (α-ENaC): 1:1,000 dilution, SPC-403D, StressMarq; β-ENaC: 1:1,000 dilution, SPC-404D, StressMarq; γ-ENaC: 1:1,000 dilution, SPC-405D, StressMarq; CYP11B2, 1:1,000 dilution, MAB6020, Merck Millipore; renal outer medullary K + channel (ROMK), 1:1,000 dilution, 20953-1-AP, Proteintech; α Na + -K + -ATPase (α-NKA), 1:1,000 dilution, ab76020, Abcam; calcium-activated potassium channel subunit α 1 (α-BK): 1:10 dilution, clone L6/60, 73-022, NeuroMab; β-actin: 1:10,000 dilution, A-2066, Sigma-Aldrich] diluted in antibody dilution buffer (1.5 g BSA, 0.1 g NaN 3 , 50 ml TBST) overnight at 4°C. .. After being washed with TBST, membranes were incubated with secondary antibodies [goat anti-rabbit/mouse horseradish peroxidase (HRP)-conjugated secondary antibody] (Thermo Scientific) for 1 h at room temperature and visualized with enhanced chemiluminescence (Thermo Scientific).

Incubation:

Concentration Assay:

Article Title: SUL-138 mitigates accelerated endothelial aging and protects the kidney
Article Snippet: .. Antibody target concentration supplier Epithelial sodium channel, α-subunit, α-ENaC 1:1000 provided by Dr. Jan Loffinga Sodium-Potassium-ATPase, α–subunit, α-Na-KATPase 1:1000 ab7671, Abcam Aquaporin 2, AQP2 1:500 ab199975, Abcam α-Tubulin 1:1000 sc5286, Santa-Cruz Epithelial sodium channel, β -subunit, β-ENaC 1:800 SPC-404D, StressMarq Epithelial sodium channel, γ -subunit, γ-ENaC 1:10,000 provided by Dr. Jan Loffinga Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 1, ERCC1 1:1000 ab129267, Abcam Glyceraldehyde-3-phosphate dehydrogenase, GAPDH 1:10,000 10494-1-AP, proteintech Cyclin-dependent kinase inhibitor 2A isoform p16INK4a, P16 1:500 51-1325GR, BD Pharmingen Cyclin-dependent kinase inhibitor 1A, P21 1:200 sc-6246, Santa-Cruz Thiazide-sensitive sodium chloride cotransporter, NCC 1:1000 SPC-402D, StressMarq Sodium-hydrogen-exchanger isoform 3, NHE3 1:1000 27190-1-AP, proteintech Phosphorylated sodium-hydrogen-exchanger isoform 3, pNHE3 1:200 sc-53961, Santa-Cruz Phosphorylated thiazide-sensitive sodium chloride cotransporter, pNCC (Thr 53) 1:500 provided by Dr. .. Rob Fentonb Bumetanide-sensitive Na-K-2Cl cotransporter, NKCC2 1:1000 SPC-401D, StressMarq Phosphorylated Bumetanide-sensitive Na-K-2Cl cotransporter, pNKCC2 1:500 Provided by Dr. Kerim Mutigc D ow nloaded from http://portlandpress.com /clinsci/article-pdf/doi/10.1042/C S20255735/978562/cs-2025-5735.pdf by guest on 28 Septem ber 2025 C linical Science.

Western Blot:

Article Title: High chloride induces aldosterone resistance in the distal nephron
Article Snippet: α‐ENaC , 3, 5 , Jan Loffing Lab , , WB , 1:3000. .. β‐ENaC , 3, 5 , Stressmarq Bioscience Inc. , SPC 404D , WB , 1:20000. .. γ‐ENaC , 3, 5 , Stressmarq Bioscience Inc. , SPC 405D , WB , 1:5000.

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